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recombinant human rh il 1β  (R&D Systems)


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    R&D Systems recombinant human rh il 1β
    A. GM-CSF human macrophages were stimulated with LPS (50 ng/mL) for 18 h in glucose-, fructose- (10 mM), or sugar-free media. <t>IL-1β,</t> IL-6, and IL-23 levels were quantified by ELISA. For IL-1β detection, 4 mM ATP was added during the final 2 h (n = 6). B. IL1B mRNA expression was analyzed by RT-qPCR under identical conditions (n = 5). C. Immunoblotting of cell lysates and supernatants from (A) was performed to assess pro- and mature (m) forms of IL-1β and caspase-1 (Casp-1). Band intensities were quantified by densitometry and normalized to β-actin (n = 5). D–E. Glycolytic activity was assessed by Seahorse extracellular flux analysis of ECAR following sequential injection of glucose, fructose (10 mM), or no sugar, followed by oligomycin (2 µM) and 2-DG (50 mM). Data represents five technical replicates (n = 3). F. HIF-1α protein and mRNA levels were measured in LPS-stimulated GM-CSF macrophages under indicated sugar conditions (n = 3–4). G–J. SKG mice were injected with curdlan to induce arthritis and administered fructose in drinking water starting 3 days prior to immunization. Glucose levels in serum and synovial fluid (SF) were assessed at day 42 (n = 5–8). H–I. mRNA levels of Hif1a and HIF-1α target genes in synovial cells were analyzed by RT-qPCR (n = 5). J. Intracellular HIF-1α expression was measured by flow cytometry in CD45⁺CD11b⁺F4/80⁺ synovial cells (n = 9–10). Graphs represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Mann–Whitney U test (A–C, E–J) or unpaired t-test (D, F).
    Recombinant Human Rh Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+%28rh%29+il-1%CE%B2+protein/bio_rxiv__64898__2026__01__26__701899-238-20-38?v=R%26D+Systems
    Average 96 stars, based on 1026 article reviews
    recombinant human rh il 1β - by Bioz Stars, 2026-08
    96/100 stars

    Images

    1) Product Images from "Fructose utilization by GM-CSF-differentiated macrophages aggravates autoimmune inflammation via MG-derived AGE–RAGE signaling"

    Article Title: Fructose utilization by GM-CSF-differentiated macrophages aggravates autoimmune inflammation via MG-derived AGE–RAGE signaling

    Journal: bioRxiv

    doi: 10.64898/2026.01.26.701899

    A. GM-CSF human macrophages were stimulated with LPS (50 ng/mL) for 18 h in glucose-, fructose- (10 mM), or sugar-free media. IL-1β, IL-6, and IL-23 levels were quantified by ELISA. For IL-1β detection, 4 mM ATP was added during the final 2 h (n = 6). B. IL1B mRNA expression was analyzed by RT-qPCR under identical conditions (n = 5). C. Immunoblotting of cell lysates and supernatants from (A) was performed to assess pro- and mature (m) forms of IL-1β and caspase-1 (Casp-1). Band intensities were quantified by densitometry and normalized to β-actin (n = 5). D–E. Glycolytic activity was assessed by Seahorse extracellular flux analysis of ECAR following sequential injection of glucose, fructose (10 mM), or no sugar, followed by oligomycin (2 µM) and 2-DG (50 mM). Data represents five technical replicates (n = 3). F. HIF-1α protein and mRNA levels were measured in LPS-stimulated GM-CSF macrophages under indicated sugar conditions (n = 3–4). G–J. SKG mice were injected with curdlan to induce arthritis and administered fructose in drinking water starting 3 days prior to immunization. Glucose levels in serum and synovial fluid (SF) were assessed at day 42 (n = 5–8). H–I. mRNA levels of Hif1a and HIF-1α target genes in synovial cells were analyzed by RT-qPCR (n = 5). J. Intracellular HIF-1α expression was measured by flow cytometry in CD45⁺CD11b⁺F4/80⁺ synovial cells (n = 9–10). Graphs represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Mann–Whitney U test (A–C, E–J) or unpaired t-test (D, F).
    Figure Legend Snippet: A. GM-CSF human macrophages were stimulated with LPS (50 ng/mL) for 18 h in glucose-, fructose- (10 mM), or sugar-free media. IL-1β, IL-6, and IL-23 levels were quantified by ELISA. For IL-1β detection, 4 mM ATP was added during the final 2 h (n = 6). B. IL1B mRNA expression was analyzed by RT-qPCR under identical conditions (n = 5). C. Immunoblotting of cell lysates and supernatants from (A) was performed to assess pro- and mature (m) forms of IL-1β and caspase-1 (Casp-1). Band intensities were quantified by densitometry and normalized to β-actin (n = 5). D–E. Glycolytic activity was assessed by Seahorse extracellular flux analysis of ECAR following sequential injection of glucose, fructose (10 mM), or no sugar, followed by oligomycin (2 µM) and 2-DG (50 mM). Data represents five technical replicates (n = 3). F. HIF-1α protein and mRNA levels were measured in LPS-stimulated GM-CSF macrophages under indicated sugar conditions (n = 3–4). G–J. SKG mice were injected with curdlan to induce arthritis and administered fructose in drinking water starting 3 days prior to immunization. Glucose levels in serum and synovial fluid (SF) were assessed at day 42 (n = 5–8). H–I. mRNA levels of Hif1a and HIF-1α target genes in synovial cells were analyzed by RT-qPCR (n = 5). J. Intracellular HIF-1α expression was measured by flow cytometry in CD45⁺CD11b⁺F4/80⁺ synovial cells (n = 9–10). Graphs represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Mann–Whitney U test (A–C, E–J) or unpaired t-test (D, F).

    Techniques Used: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot, Activity Assay, Injection, Flow Cytometry, MANN-WHITNEY



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    A. GM-CSF human macrophages were stimulated with LPS (50 ng/mL) for 18 h in glucose-, fructose- (10 mM), or sugar-free media. <t>IL-1β,</t> IL-6, and IL-23 levels were quantified by ELISA. For IL-1β detection, 4 mM ATP was added during the final 2 h (n = 6). B. IL1B mRNA expression was analyzed by RT-qPCR under identical conditions (n = 5). C. Immunoblotting of cell lysates and supernatants from (A) was performed to assess pro- and mature (m) forms of IL-1β and caspase-1 (Casp-1). Band intensities were quantified by densitometry and normalized to β-actin (n = 5). D–E. Glycolytic activity was assessed by Seahorse extracellular flux analysis of ECAR following sequential injection of glucose, fructose (10 mM), or no sugar, followed by oligomycin (2 µM) and 2-DG (50 mM). Data represents five technical replicates (n = 3). F. HIF-1α protein and mRNA levels were measured in LPS-stimulated GM-CSF macrophages under indicated sugar conditions (n = 3–4). G–J. SKG mice were injected with curdlan to induce arthritis and administered fructose in drinking water starting 3 days prior to immunization. Glucose levels in serum and synovial fluid (SF) were assessed at day 42 (n = 5–8). H–I. mRNA levels of Hif1a and HIF-1α target genes in synovial cells were analyzed by RT-qPCR (n = 5). J. Intracellular HIF-1α expression was measured by flow cytometry in CD45⁺CD11b⁺F4/80⁺ synovial cells (n = 9–10). Graphs represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Mann–Whitney U test (A–C, E–J) or unpaired t-test (D, F).
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    A. GM-CSF human macrophages were stimulated with LPS (50 ng/mL) for 18 h in glucose-, fructose- (10 mM), or sugar-free media. <t>IL-1β,</t> IL-6, and IL-23 levels were quantified by ELISA. For IL-1β detection, 4 mM ATP was added during the final 2 h (n = 6). B. IL1B mRNA expression was analyzed by RT-qPCR under identical conditions (n = 5). C. Immunoblotting of cell lysates and supernatants from (A) was performed to assess pro- and mature (m) forms of IL-1β and caspase-1 (Casp-1). Band intensities were quantified by densitometry and normalized to β-actin (n = 5). D–E. Glycolytic activity was assessed by Seahorse extracellular flux analysis of ECAR following sequential injection of glucose, fructose (10 mM), or no sugar, followed by oligomycin (2 µM) and 2-DG (50 mM). Data represents five technical replicates (n = 3). F. HIF-1α protein and mRNA levels were measured in LPS-stimulated GM-CSF macrophages under indicated sugar conditions (n = 3–4). G–J. SKG mice were injected with curdlan to induce arthritis and administered fructose in drinking water starting 3 days prior to immunization. Glucose levels in serum and synovial fluid (SF) were assessed at day 42 (n = 5–8). H–I. mRNA levels of Hif1a and HIF-1α target genes in synovial cells were analyzed by RT-qPCR (n = 5). J. Intracellular HIF-1α expression was measured by flow cytometry in CD45⁺CD11b⁺F4/80⁺ synovial cells (n = 9–10). Graphs represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Mann–Whitney U test (A–C, E–J) or unpaired t-test (D, F).
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    A. GM-CSF human macrophages were stimulated with LPS (50 ng/mL) for 18 h in glucose-, fructose- (10 mM), or sugar-free media. IL-1β, IL-6, and IL-23 levels were quantified by ELISA. For IL-1β detection, 4 mM ATP was added during the final 2 h (n = 6). B. IL1B mRNA expression was analyzed by RT-qPCR under identical conditions (n = 5). C. Immunoblotting of cell lysates and supernatants from (A) was performed to assess pro- and mature (m) forms of IL-1β and caspase-1 (Casp-1). Band intensities were quantified by densitometry and normalized to β-actin (n = 5). D–E. Glycolytic activity was assessed by Seahorse extracellular flux analysis of ECAR following sequential injection of glucose, fructose (10 mM), or no sugar, followed by oligomycin (2 µM) and 2-DG (50 mM). Data represents five technical replicates (n = 3). F. HIF-1α protein and mRNA levels were measured in LPS-stimulated GM-CSF macrophages under indicated sugar conditions (n = 3–4). G–J. SKG mice were injected with curdlan to induce arthritis and administered fructose in drinking water starting 3 days prior to immunization. Glucose levels in serum and synovial fluid (SF) were assessed at day 42 (n = 5–8). H–I. mRNA levels of Hif1a and HIF-1α target genes in synovial cells were analyzed by RT-qPCR (n = 5). J. Intracellular HIF-1α expression was measured by flow cytometry in CD45⁺CD11b⁺F4/80⁺ synovial cells (n = 9–10). Graphs represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Mann–Whitney U test (A–C, E–J) or unpaired t-test (D, F).

    Journal: bioRxiv

    Article Title: Fructose utilization by GM-CSF-differentiated macrophages aggravates autoimmune inflammation via MG-derived AGE–RAGE signaling

    doi: 10.64898/2026.01.26.701899

    Figure Lengend Snippet: A. GM-CSF human macrophages were stimulated with LPS (50 ng/mL) for 18 h in glucose-, fructose- (10 mM), or sugar-free media. IL-1β, IL-6, and IL-23 levels were quantified by ELISA. For IL-1β detection, 4 mM ATP was added during the final 2 h (n = 6). B. IL1B mRNA expression was analyzed by RT-qPCR under identical conditions (n = 5). C. Immunoblotting of cell lysates and supernatants from (A) was performed to assess pro- and mature (m) forms of IL-1β and caspase-1 (Casp-1). Band intensities were quantified by densitometry and normalized to β-actin (n = 5). D–E. Glycolytic activity was assessed by Seahorse extracellular flux analysis of ECAR following sequential injection of glucose, fructose (10 mM), or no sugar, followed by oligomycin (2 µM) and 2-DG (50 mM). Data represents five technical replicates (n = 3). F. HIF-1α protein and mRNA levels were measured in LPS-stimulated GM-CSF macrophages under indicated sugar conditions (n = 3–4). G–J. SKG mice were injected with curdlan to induce arthritis and administered fructose in drinking water starting 3 days prior to immunization. Glucose levels in serum and synovial fluid (SF) were assessed at day 42 (n = 5–8). H–I. mRNA levels of Hif1a and HIF-1α target genes in synovial cells were analyzed by RT-qPCR (n = 5). J. Intracellular HIF-1α expression was measured by flow cytometry in CD45⁺CD11b⁺F4/80⁺ synovial cells (n = 9–10). Graphs represent mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Mann–Whitney U test (A–C, E–J) or unpaired t-test (D, F).

    Article Snippet: Cells were plated in 96-well U-bottom plates and stimulated with anti-CD3/CD28-coated beads for 7 days under the following cytokine conditions: recombinant human (rh) IL-1β (30 ng/mL), rhIL-6 (30 ng/mL), rhIL-23 (10 ng/mL), and rhTGF-β (10 ng/mL) (all from R&D Systems, Minneapolis, MN) for Th17 polarization, rhIL-2 (100 IU/mL; PeproTech) and rhIL-12 (10 ng/mL; R&D Systems) for Th1 polarization, and rhIL-2 (100 IU/mL) alone for non-polarizing control.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot, Activity Assay, Injection, Flow Cytometry, MANN-WHITNEY

    (A) mRNA expression of IL-1β and IL-1RA was analyzed by the UALCAN tool. (B) Co-expression between IL-1β and IL-1RA analyzed by the GEPIA tool. (C) Protein expression of IL-1β and IL-1RA confirmed by IHC. (D) Protein expression of IL-1β and IL-1RA confirmed by western blotting. (E) Association etween IL-1β and OS/RFS analyzed by the GEPIA tool. IL, interleukin; OS, overall survival; RFS, recurrence-free survival.

    Journal: Oncology Reports

    Article Title: Interleukin 1β/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy

    doi: 10.3892/or.2020.7475

    Figure Lengend Snippet: (A) mRNA expression of IL-1β and IL-1RA was analyzed by the UALCAN tool. (B) Co-expression between IL-1β and IL-1RA analyzed by the GEPIA tool. (C) Protein expression of IL-1β and IL-1RA confirmed by IHC. (D) Protein expression of IL-1β and IL-1RA confirmed by western blotting. (E) Association etween IL-1β and OS/RFS analyzed by the GEPIA tool. IL, interleukin; OS, overall survival; RFS, recurrence-free survival.

    Article Snippet: Recombinant human (rh) IL-1β protein and IL-1RA protein were purchased from R&D Systems, Inc.

    Techniques: Expressing, Western Blot

    (A) EMT-associated markers detected by western blotting and RT-qPCR. (B) Autophagy-associated markers detected by western blotting and RT-qPCR. (C) EMT-associated markers detected by western blotting and RT-qPCR after treatment of RAPA in the rhIL-1β group. *P<0.05, **P<0.01 and ***P<0.001. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription quantitative PCR; RAPA, rapamycin rh, recombinant human, IL, interleukin.

    Journal: Oncology Reports

    Article Title: Interleukin 1β/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy

    doi: 10.3892/or.2020.7475

    Figure Lengend Snippet: (A) EMT-associated markers detected by western blotting and RT-qPCR. (B) Autophagy-associated markers detected by western blotting and RT-qPCR. (C) EMT-associated markers detected by western blotting and RT-qPCR after treatment of RAPA in the rhIL-1β group. *P<0.05, **P<0.01 and ***P<0.001. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription quantitative PCR; RAPA, rapamycin rh, recombinant human, IL, interleukin.

    Article Snippet: Recombinant human (rh) IL-1β protein and IL-1RA protein were purchased from R&D Systems, Inc.

    Techniques: Western Blot, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction, Recombinant

    (A) Images of the subcutaneous xenografts. (B) Growth curve of xenografts in rhIL-1β and control groups. (C) Weight of the rhIL-1β and control groups before and after treatment. **P<0.01, ***P<0.001. rh, recombinant human; IL, interleukin.

    Journal: Oncology Reports

    Article Title: Interleukin 1β/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy

    doi: 10.3892/or.2020.7475

    Figure Lengend Snippet: (A) Images of the subcutaneous xenografts. (B) Growth curve of xenografts in rhIL-1β and control groups. (C) Weight of the rhIL-1β and control groups before and after treatment. **P<0.01, ***P<0.001. rh, recombinant human; IL, interleukin.

    Article Snippet: Recombinant human (rh) IL-1β protein and IL-1RA protein were purchased from R&D Systems, Inc.

    Techniques: Control, Recombinant